Identification and elimination of target-related matrix interference in a neutralizing anti-drug antibody assay

Understanding the Supersensitive Anti-Drug Antibody Assay: Unexpected High Anti-Drug Antibody Incidence and Its Clinical Relevance

Numbers of biotherapeutic products in development have increased over past decade. Despite providing significant benefits to patients with unmet needs, almost all protein-based biotherapeutics could induce unwanted immunogenicity, which result in a loss of efficacy and/or increase the risk of adverse reactions, such as infusion reactions, anaphylaxis, and even life-threatening response to endog...

Titration of Rinderpest Virus and Its Neutralizing Antibody

Decreased T Cell Response to Mitogen and Increased Anti-cytoplasmic Antibody in Drug-Free Schizophrenic Patients

Background: Apart from genetic and environmental factors, activation of autoreactive mechanisms has been proposed to play a role in the pathogenesis of schizophrenia. In re-cent years, considerable work has been carried out to understand the role and contribution of the immune system in this disease. Objective: To investigate the T cell response to phytohaemagglutinin (PHA) and determine the se...

Anti‐CD22 and anti‐CD79b antibody‐drug conjugates preferentially target proliferating B cells

BACKGROUND AND PURPOSE CD22 and CD79b are cell-surface receptors expressed on B-cell-derived malignancies such as non-Hodgkin's lymphoma (NHL). An anti-mitotic agent, monomethyl auristatin E, was conjugated to anti-CD22 and anti-CD79b antibodies to develop target-specific therapies for NHL. The mechanism of action (MOA) and pharmacological and pharmacokinetic (PK) profiles of these antibody-dru...

Misleading FT4 measurement: Assay-dependent antibody interference

Introduction: Commonly used free thyroxine (FT4) immunoassays can be falsely elevated due to interference causing misinterpreted thyroid function. We present two cases with high FT4 concentrations due to antibody interference. This study’s aim was to investigate the source of the FT4 immunoassay interference and possibility of its removal by two different techniques in order to correct the disc...